production and purification of a protease from an alkalophilic bacillus sp. 2-5 strain isolated from soil
نویسندگان
چکیده
this research has focused on isolation and characterization of a strain of bacillus sp. from alkaline soil, which was able to produce extracellular alkaline protease at phs ranging from 8 to 11 and temperatures of 20 to 50ºc. also the impact of different carbon andnitrogen sources were investigated. the yield and fold of enzyme purification was 24% and 50 times, respectively. molecular weight of purified enzyme was measured by sds-page as 24.7 kda. the alkaline protease produced by bacillus sp. 2 - 5 showed the most caseinolytic activity (without any gelatinolytic activity)at ph>10
منابع مشابه
Production and purification of a protease from an alkalophilic Bacillus sp. 2-5 strain isolated from soil
This research has focused on isolation and characterization of a strain of Bacillus <span style="font-variant: normal; font-style: normal; font-family: ArialMT...
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عنوان ژورنال:
iranian journal of biotechnologyناشر: national institute of genetic engineering and biotechnology
ISSN 1728-3043
دوره 5
شماره 2 2007
کلمات کلیدی
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